Objective To establish an endometriosis rat model by autotransplanting the endometrium in three different sites of the same rat, and to compare the effects of modeling. Methods The endometrium tissues of 40 adult unmated female SD rats were autotransplanted into the ovary, the uterosacral ligament, and the abdominal wall. Transplants were collected for volume and weight determination and pathological examination on the 28^th day after surgery. The successful modeling rates of transplanting endometrium tissues to three different sites were compared. Results The volume and weight of transplants in the ovarian were greater than those in the other two sites (P<0.05), and the transplants on uterosacral ligament and abdominal wall had no notable difference in the volume or weight. The successful modeling rates of the three sites had no significant difference, and the transplants had similar histopathology. Conclusion Autotransplantation of endometriosis in the ovary, the uterosacral ligament, and the abdominal wall of rats can all establish the endometriosis model, and the pathological changes are similar to endometriosis patients; the best modeling effect is seen in the ovary site, which is more conducive to new treatments for endometriosis.