Electronic pacemakers has become the preferred treatment for arrhythmia, especially for severe bradycardiac arrhythmia. Although the technology of electronic pacemaker was gradually improving, it still has some shortcomings. Over the past 10 years, research has confirmed that biological pacemaker can be created by gene therapy and cell therapy; with the development of molecular biology and cell biology, biological pacemaking technology has been continuously broken. Currently there are two approaches to construct biological pacemakers:one is to introduce pacemaker genes into mesenchymal stem cells, and the other is to induce pluripotent stem cells to sinoatrial node cells. In this paper, we reviewed the latest research progress of biological pacemakers, so as to analyze the existing problems in biological cardiac pacing and future research directions.