Widespread distribution, strong toxicity, and difficulty to detoxicate make marine biotoxins a great threat to the nation's public security and health. To meet this threat, great endeavor has been devoted to marine biotoxin analysis. Up to now the methods for marine biotoxins analysis include bioassay test, immunoassay, liquid chromatography, enzyme activity assay, cytotoxicity test, etc. In this review, we summarized the major technique developments in marine biotoxins analysis.