Objective To investigate the feasibility and advantages of five skills summarized by relative motion theory in reducing difficulty of suturing the kidney during retroperitoneal laparoscopic partial nephrectomy. Methods We retrospectively analyzed the clinical data of 89 patients undergoing retroperitoneal laparoscopic partial nephrectomy in the Department of Urology of Changhai Hospital, Second Military Medical University between Jan. 2014 and May 2016. There were 42 cases in the control group and 47 cases in the skill group. Five skills including "move, add, push, pull, and shift" were strictly applied to reconstruct the kidney in the skill group, but not fully used in the control group. Warm ischemic time, operation time, intra-operative blood loss, renal function and complications were compared between the two groups during and after operation. Results The operations were successfully completed in all the 89 cases. The average warm ischemic time was (22.4±4.9) min and the operation time was (96.0±11.6) min in the skill group; while those in the control group were (24.5±4.8) min and (102.0±13.7) min, respectively, and the differences between the two groups were statistically significant (P<0.05). There were no significant differences in the intra-operative blood loss, blood transfusion or artery injury between the two groups. No complications such as urine leakage or post-operative bleeding were observed in either groups. Conclusion The five skills summarized by relative motion theory are clinically feasible and safe in retroperitoneal laparoscopic partial nephrectomy, and can shorten operation time and renal warm ischemic time.