Objective To design a new method for visual acuity test naming computerized tumbling E test, and to explore the application of computerized tumbling E test. Methods The feasibility of computerized tumbling E test was investigated by computer simulation experiment. Based on the simulation experiment, a total of 30 subjects ([26.1±4.7] years old, 18 males and 12 females) received binocular vision acuity examination by computerized tumbling E test with 40 trials or 80 trials and standard logarithmic visual acuity chart (random sequence), respectively. The accuracy of computerized tumbling E test was evaluated by comparing the consistency limit with standard logarithmic visual acuity chart. The correlation and difference between the two tests were evaluated by paired samples t test. Results There was no significant difference between the results of the computerized tumbling E test with 40 trials and standard logarithmic visual acuity chart,while the difference between the results of the 80 trials and standard logarithmic visual acuity chart was statistically significant (P<0.05). Conclusion The consistency is good for adult visual test using the computerized tumbling E test with reasonable number of trials (40 trials) and the standard logarithmic visual acuity chart. Compared with the standard logarithmic visual acuity chart, computerized tumbling E test has advantages such as avoiding the problem of optotype memory and reducing the visual crowding effect, showing practical application value (P<0.05).