Objective Discussion on the application value of a cooling module of paraffin section in pathological section.Methods Random selection of 100 paraffin blocks, the pathology technicians first roughed 100 wax pieces and then set them aside. The 100 pieces of wax were respectively sliced up and cooled with ice block made by themselves, frozen table and cooling module. The sections were successively divided into group A, group B and group C, and dyed according to the same program. The scores of the three indexes, such as thickness uniformity, no wrinkles and no crack, were evaluated by a 10-point scale, respectively.The three groups were compared with each other. The differences between the 3 groups of slices in timeliness（carry on 10 tests）and capital input were also analyzed. Results ①There were statistically significant differences in the scores of thickness uniformity, no wrinkles and no crack index(P＜0.01) between group A and group B. There was significant difference in the score of no wrinkle index(P＜0.01) while there were no significant difference in the scores of thickness uniformity or no crack index (P＞0.05)between group A and C.There were statistically significant differences in the scores of thickness uniformity, no wrinkles and no crack index(P＜0.01) between group B and group C. ② Group A slices took 65.30±1.44 minutes, group B slices took 79.80±1.95 minutes, and group C slices took 62.50±2.17 minutes. The difference between group A and B was statistically significant(t=5.977，P＜0.01). The difference between group B and C was statistically significant(t=5.930，P＜0.01). There was no statistical difference between group A and C (t=1.007,P＞0.05).③The cost of group B in six years was about 7000 yuan.The cost of group A that the ice made by themselves in six years was about 3500 yuan for a refrigerator, a stainless steel plate, and water. The cost of paraffin section cooling module of group C was the same as that of group A, less than that of group B. Conclusion In the process of pathological routine paraffin section, the cooling module of paraffin section was used to slice the wax block after cooling, which had the advantages of good slice quality, high efficiency, simple operation and so on, which was worthy of recommendation.