Objective To investigate the preliminary application of the da Vinci Si robotic surgery system for gynecological single-port laparoscopic surgery. Methods Five patients who were hospitalized for elective gynecological surgery in the Department of Obstetrics and Gynecology of Changzheng Hospital, Naval Medical University (Second Military Medical University) from May 27 to June 20, 2018 were enrolled. The single-port laparoscopic surgeries were performed with the third-generation da Vinci Si robotic surgery system and Lagiport^TM multi-channel single-port laparoscopic puncture device. One patient with high-grade cervical intraepithelial neoplasia (microinfiltration cannot be excluded after cervical conization) underwent total hysterectomy+sentinel lymphadenectomy; one patient with stageⅠa1 invasive cervical carcinoma underwent total hysterectomy+bilateral adnexectomy+sentinel lymphadenectomy; one patient with stageⅠa1 endometrial carcinoma underwent total hysterectomy+bilateral adnexectomy+sentinel lymphadenectomy; one patient with stage Ⅳ endometriosis underwent extensive enterolysis+unilateral adnexectomy; and one patient with stageⅡa1 invasive cervical carcinoma underwent extensive hysterectomy+bilateral adnexectomy+pelvic lymph node dissection. Results The operation of the 5 patients was successfully completed without additional auxiliary port. The operative duration of the 5 patients were 174, 110, 90, 125 and 300 min, and the volumes of the intraoperative blood loss were about 110, 80, 100, 210 and 150 mL. None of the 5 patients had perioperative complications. Conclusion The third-generation da Vinci Si robotic surgery system has promising feasibility for gynecological single-port laparoscopic surgery.