Objective To investigate the clinical outcome of laparoscopic extraperitoneal uterine suspension for uterine prolapse. Methods A total of 66 patients with uterine prolapse who underwent laparoscopic extraperitoneal uterine suspension from February 2011 to March 2018 in our hospital were enrolled in this study. Therapeutic effects were retrospectively analyzed. Results All the operation was successfully completed. The mean operation time was (78.64±36.23) min, the mean bleeding volume was (80.00±28.62) mL, and the mean hospitalization time was (8.27±3.31) d. One case (1.52%) had poor healing of abdominal incision. One case (1.52%) had erosion of material exposure. No deep vein thromboembolism was found. Clinical symptoms and life quality of all patients were significantly improved. The location of Aa, Ba, C, D, Ap, and Bp in the Pelvic Organ Prolapse Quantitation (POP-Q) system at 3 months after surgery were significantly different from preoperative ones (all P<0.01). Conclusion Laparoscopic extraperitoneal uterine suspension is simple and practicable for prolapse. It has the advantages of significant effect, small trauma, quick recovery, and low incidence of complications, and can effectively alleviate clinical symptoms and improve life quality of patients.