Objective To explore the post-setting and clinical practice of stroke emergency nurse. Methods Through selecting stroke emergency nurses, systematic training and assessing stroke first-aid theory and skills, we set up a new post in our hospital called stroke emergency nurse and developed a series of duties and procedures of work. Results The stroke emergency nurses with undergraduate degree were required to have more than 2 years of work experience in the Emergency, Neurosurgery, Cerebrovascular Surgery, Interventional Operation Room, Rehabilitation Department, and those with college education were required to have more than 5 years of work experience. A total of 32 trainees participated in the training. The theoretical assessment scores increased from 73.75±11.73 before training to 84.31±8.63 after training, the assessment scores of thrombolytic drug use increased from 82.91±2.75 to 92.69±2.78, the assessment scores of simulated stroke test increased from 82.25±3.81 to 92.56±2.94, and the assessment scores of interventional nursing training increased from 82.44±3.59 to 92.00±2.93; and the differences were statistically significant (t=14.91, 21.09, 14.34 and 16.39; all P<0.01). The numbers of excellent, good, qualified and unqualified trainees in the theoretical examination of hyperacute and acute stroke before training were 4, 6, 17 and 5, respectively, and the numbers after training were 9, 14, 9 and 0, respectively; and the difference was statistically significant (χ²=12.585, P=0.006). Conclusion It is clinically effective and feasible to set up a post of stroke emergency nurse through clinical screening, theoretical training, and clinical training. However, the training and post promotion mechanism need to be further improved.