Objective To explore the safety and feasibility of applying self-made peritoneal reflexed suspension device in retroperitoneoscopic partial nephrectomy for ventral renal tumors. Methods From Jan. 2018 to Jun. 2018, 15 patients with ventral renal tumors were treated by retroperitoneoscopic partial nephrectomy in Department of Urology of Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University). The self-made simple peritoneal retrorse suspension device was applied during the operation, and it consisted of the Hem-o-lok clamp and the associated puncture device, fixation device and clipper. Fifteen patients, aged (62.5±9.2) years, included 9 males and 6 females. All cases had single tumors located on the ventral side of the kidney, with an average maximum diameter of (2.9±1.0) cm. The clinical data, including intraoperative blood loss, operation time, renal warm ischemia time, average postoperative hospital stay and so on, were statistically analyzed. Results Retroperitoneoscopic partial nephrectomy was successfully performed on the 15 patients. The average operation time of 15 patients was (88.9±24.5) min, average renal warm ischemia time was (21.4±4.6) min, average intraoperative blood loss was (72.0±16.6) mL, and average hospital stay was (6.9±1.2) d. None of the 15 patients had blood transfusion or major surgical complications during or after operation. Conclusion This self-made peritoneal retrorse suspension device is safe and feasible in retroperitoneoscopic partial nephrectomy for ventral renal tumors, and is easy to operate during the operation. Meanwhile, it has the advantages of economy and convenience and is worthy of being popularized in clinical practice.