Objective To study the expression of transient receptor potential cation channel subfamily C member 6 (TRPC6) in endometrial carcinoma tissues and their role in regulating cell cycle of endometrial carcinoma cells. Methods Quantitative real-time PCR and Western blotting were used to examine the expression of TRPC6 in 30 normal endometrial specimens, 30 atypical hyperplasia specimens and 32 endometrial carcinoma specimens. SKF96365 (an inhibitor of TRPC6 channel) and RNA interference (RNAi) targeting TRPC6 by small interference RNA (siRNA) were used to block TRPC6 so as to explore the role of TRPC6 in regulating the cell cycle of endometrial carcinoma cells HEC-1A. Results The expression levels of TRPC6 mRNA and protein in endometrial carcinoma were significantly higher than those in the atypical hyperplasia endometria and normal endometrial tissues (P<0.01). SKF96365 retarded cell cycle at G₂/M phase in a dose-dependent manner and reduced HEC-1A cells of G₀/G₁ phase. Transfection with target-TRPC6 siRNA retarded cell cycle of HEC-1A cells at G₂/M phase, and reduced HEC-1A cells of G₀/G₁ phase compared with negative control siRNA. Meanwhile, transfection with target-TRPC6 siRNA increased phosphorylated cell division cycle 2 (pCDC2) protein expression in HEC-1A cells. Conclusion The expression of TRPC6 is elevated in endometrial carcinoma tissues. TRPC6 may influence cell cycle through regulating pCDC2.