Objective To introduce our experience on three-step anatomical bladder neck preservation in robot-assisted laparoscopic radical prostatectomy (RARP). Methods A retrospective analysis was conducted on the clinical data of 30 patients with prostate tumors, who were admitted to our hospital and received three-step anatomical bladder neck preservation in RARP from Dec. 2017 to Dec. 2018. The procedures of three-step anatomical bladder neck preservation were as follows:(1) locate the bladder neck-prostate junction, separate the surface fatty tissue, and expose the anterior wall of urethra; (2) dissect from the loose tissue layer between the trigone of bladder and prostate to the deep layer, expose the anterior layer of seminal vesicle, then expose the lateral wall of urethra along this layer; (3) dissect and expose the posterior wall of urethra. The success rate of bladder neck preservation, the time of bladder neck preservation and the incidence of adverse events were observed and recorded. Results All the 30 patients received three-step anatomical bladder neck preservation in RARP. The success rate of anatomical bladder neck preservation was 93.3% (28/30). The bladder neck failed to be completely preserved in two patients because of the irregular protrusion of the middle lobe of prostate to the bladder. The time of bladder neck preservation was 8-10 min, with an average of (9.2±0.8) min. No adverse events occurred. Conclusion The three-step anatomical bladder neck preservation in RARP has a high success rate and is worth promoting.