Objective To investigate the feasibility, safety and short-term recovery of urinary continence of the transvesical robot-assisted laparoscopic radical prostatectomy (RARP) for treatment of prostate cancer after holmium laser enucleation of prostate (HoLEP). Methods From Aug. 2016 to Apr. 2019, 12 patients confirmed with post-HoLEP prostatic cancer underwent transvesical RARP. The age of the patients ranged from 60 to 69 years. The preoperative prostate-specific antigen (PSA) was 1.5-8.7 ng/mL. The post-HoLEP Gleason score was ≤ 7 in all the 12 patients and preoperative TNM stage ranged from T1a-T1b. All patients were diagnosed as having prostate cancer by pathology after HoLEP, and none of them received endocrine therapy. The operation time, intraoperative blood loss and length of hospital stay were recorded. Results The operations of all the 12 cases were successfully completed without conversion to open surgery. The operation time was (116.2±15.1) min (115-130 min), the estimated blood loss was (125.1±20.3) mL (80-210 mL), and the postoperative hospital stay was (6.7±1.7) d (5-9 d). The catheter was removed 7 days after operation. No serious complications occurred. Eleven patients (91.7%) had immediate recovery of urinary continence, and one patient had no urine leakage after 2 weeks. Postoperative pathology showed that Gleason score was 6 in eight patients, 7 in two patients, 8 in one patient, and cancer tissue was not found in one patient. One patient with Gleason score of 8 was positive on the surgical margin at the apex of prostate. During 3 to 30 months of follow-up, all patients regained urinary continence 3 months postoperatively. Conclusion Early HoLEP surgery may increase the difficulty of radical prostatectomy. The transvesical RARP is a safe and technically feasible treatment for prostate cancer after HoLEP. The anatomical structure around the prostate associated with urinary continence is preserved as much as possible while achieving the goal of radical resection. The urethral catheter can be removed shortly after surgery and the urinary continence can be restored early.