Objective To explore the safe time limit of apneic anesthesia with intermittent ventilation (AAIV) for children receiving resection of laryngeal papillomatosis. Methods The anesthesia records of 27 children diagnosed with laryngeal papillomatosis and underwent surgery with AAIV in our hospital from 2015 to 2019 were analyzed retrospectively. The data of gender, age, disease diagnosis, body weight, height, hemoglobin level, anesthesia time, operation time, and recovery time were obtained from the medical records, and the theoretical calculation model was constructed to calculate the theoretical safe time limit and CO₂ accumulation. The results were compared with the existing literatures. Results No serious complications such as severe hypoxia and delayed recovery were recorded during anesthesia and resuscitation, and the perioperative vital signs were stable in all patients. According to the calculation, the theoretical apnea time limit was 247-360 s (4.12-6.00 min) in the model patient, and the theoretical partial pressure of carbon dioxide at end-trial (P_ETCO₂) reached 74.26 mmHg (1 mmHg=0.133 kPa). The average time limit of apnea in the retrieved literatures was 224-292 s (3.73-4.87 min), and the average arterial partial pressure of carbon dioxide (PaCO₂) or P_ETCO₂ was within 70 mmHg. The calculated data of the model were close to the clinical experience data reported in the existing literatures but not completely consistent. Conclusion AAIV for laryngeal papilloma removal in children has corresponding theoretical evidence. However, in practical applications, for children with dysplasia, respiratory tract infections or other diseases which can affect the lung reserve function, it is recommended to take reduction of pulse oxygen saturation (SpO₂) to 95% as an indication to restore ventilation, and we always need to pay attention to CO₂ accumulation.