Objective To investigate the clinical value of Mayo adhesive probability (MAP) score in the preoperative evaluation of robot-assisted laparoscopic partial nephrectomy (RAPN). Methods The clinical data of 229 patients with T1aN0M0 renal tumor who received RAPN by the same surgeon in Changzheng Hospital of Naval Medical University (Second Military Medical University) from Oct. 2016 to Oct. 2018 were retrospectively analyzed. There were 145 males and 84 females, with an average age of (53.14±11.84) years, including 122 cases of left renal tumor and 107 cases of right renal tumor. The preoperative estimated glomerular filtration rate (eGFR) was (100.24±24.35) mL/(min·1.73 m²). The patients were divided into two groups according to the MAP score:low-MAP group (MAP score ≤ 3, n=175) and high-MAP group (MAP score>3, n=54). The clinical data were compared between the two groups. Results The RAPN was successfully performed in all the 229 patients, with no intraoperative conversion to radical nephrectomy or open surgery. The operation time was (140.57±41.05) min, the intraoperative blood loss was (98.56±65.38) mL, the total transfusion rate was 7.9% (18/229), and the postoperative hospital stay was (6.41±2.39) days. Four patients had no renal artery blocking, 13 patients had selective branch artery blocking, and the rest patients had main renal artery blocking. The warm ischemia time of kidney was (17.73±7.91) min. Thirteen patients with Clavien-Dindo classification grade Ⅱ received perioperative blood transfusion therapy, four patients received digital subtraction angiography embolization for hemostasis due to postoperative hemorrhage, and one patient underwent emergency radical nephrectomy due to hemorrhage. All patients were discharged uneventfully after treatment. During a 1-year follow-up, no tumor recurrence or metastasis was found. The eGFR at the latest follow-up was (94.40±22.63) mL/(min·1.73 m²), significantly different from preoperation (P=0.001). Compared with the low-MAP group, the high-MAP group had significantly longer operation time ([152.51±39.53] min vs [136.91±41.15] min, P=0.015), more intraoperative blood loss ([123.11±93.15] mL vs [94.75±59.89] mL, P=0.029), higher Clavien-Dindo classification (45 cases [83.3%] in grade Ⅰ, eight [14.8%] in grade Ⅱ and one [1.9%] in grade Ⅲ vs 165 [94.3%] in grade Ⅰ, seven [4.0%] in grade Ⅱ and three [1.7%] in grade Ⅲ, P=0.019), and longer postoperative hospital stay ([7.04±3.32) d vs [6.21±2.01] d, P=0.027). Conclusion MAP score can be used to evaluate the risk of prolonged kidney and tumor dissociation time and increased bleeding in RAPN in advance, guiding clinicians to make better preoperative surgical plan.