Objective To screen the differentially expressed long non-coding RNAs (lncRNAs) in glioblastoma, and to explore its role in the growth and invasion of glioblastoma. Methods Eight matched glioblastoma tumor tissues and peritumoral tissues were selected for high-throughput hybridization experiments, and the lncRNA with largest expression difference was screened. Human glioblastoma cell models with overexpression and interference expression of the lncRNA were established. Cell counting kit-8 (CCK-8) was used to detect the cell proliferation ability, flow cytometry was used to detect the apoptosis level, and Transwell assay was used to detect the cell invasion and migration abilities. Results The lncRNA MIR210HG, which was highly expressed in glioblastoma tissues, was screened out, and the glioblastoma cell models with overexpression and interference expression of lncRNA MIR210HG were successfully constructed. In vitro experiments showed that after overexpression of lncRNA MIR210HG, the apoptosis level of the glioblastoma cells was decreased, and the proliferation, invasion and migration abilities were increased, and after interference expression of lncRNA MIR210HG, the apoptosis level of the glioblastoma cells was increased, and the proliferation, invasion and migration abilities were decreased, with significant differences compared with control group (all P<0.05). Conclusion LncRNA MIR210HG can inhibit the apoptosis of glioblastoma cells, and promote cell proliferation, invasion and migration; and it may serve as a new target of glioblastoma therapy.