Objective To explore the feasibility of two-dimensional phase contrast magnetic resonance imaging (2D PC-MRI) for quantitatively monitoring the pulmonary arterial hemodynamics in normal population, and evaluate the influence of different levels of smoking status on pulmonary hemodynamics in normal population. Methods A total of 49 normal male smoking volunteers who underwent pulmonary artery 2D PC-MRI examinations in our hospital from May 2019 to Jan. 2020 were included in the study, and smoking parameters (smoking intensity, smoking time, and smoking burden) were recorded. The hemodynamic parameters of the main pulmonary artery were measured by 2D PC-MRI, including positive peak velocity (PPV), negative peak velocity (PNV), average blood flow (AF), average positive blood flow (APF) and average negative blood flow (ANF). Intraclass correlation coefficient (ICC) was used to analyze the intra- and inter-group consistency of hemodynamic parameters. The correlation between smoking parameters and hemodynamic parameters was analyzed. The differences of hemodynamic parameters between non-heavy smoking group (smoking time <20 years, n=24) and heavy smoking group (smoking time ≥ 20 years, n=25) were also analyzed. Results The intra- and inter-group ICC values of PPV, PNV, AF, APF and ANF were 0.890-0.987, indicating good consistency. Smoking time was negatively correlated with PPV, AF and APF, respectively (r=-0.321, -0.350, -0.311; P=0.024, 0.014, 0.029). PPV, AF and APF of the heavy smoking group were significantly lower than those of the non-heavy smoking group (all P<0.05). Conclusion The 2D PC-MRI technology can be used to monitor the influence of different smoking degrees on the main pulmonary hemodynamics in normal population. With the prolongation of smoking time, the main pulmonary artery positive flow parameters (PPV, AF and APF) decrease, especially in heavy smokers.