Objective To establish a training system for robot-assisted laparoscopic partial nephrectomy (RAPN) based on virtual reality technology and segmented operation concept. Methods A urologist who has recently been qualified for da Vinci robotic surgery was selected to receive basic operation training and suture strengthening training using virtual reality technology. The procedures of the RAPN were divided into six steps. The trained doctor completed one step each time in the actual operation, and gained practical operation experience with minimal impact on patients. Finally, the trained urologist tried to carry out RAPN independently. Results The trained urologist successfully completed the virtual reality training, and participated in 18 cases of actual operation during the segmented operation. Compared with the operation carried out by superior doctor alone, the average operation time of the segmented operation was prolonged from (134±41) min to (161±51) min, but the difference was not statistically significant (P=0.087). There was no significant difference in perioperative indexes such as estimated blood loss, incidence of complications or postoperative hospital stay. After training, the trained urologist was able to carry out RAPN independently. Conclusion With the help of virtual reality training and segmented operation, the safety and efficacy of the RAPN can be guaranteed, and the trained urologist can complete the operation with high efficiency and quality.