Objective To explore the effects of delayed umbilical cord clamping (DCC) on early jaundice and intracranial hemorrhage in moderate and late preterm infants. Methods Moderate and late preterm (32-36⁺⁶ weeks) infants, who were vaginal delivered from Feb. 1, 2016 to Jan. 31, 2018 in Maternal and Child Health Care Hospital of Shanghai Jiading District, were selected, and were divided into early umbilical cord clamping (ECC) group (who were born during Feb. 1, 2016 to Jan. 31, 2017 with umbilical cord clamping in 15 s after delivery) and DCC group (who were born during Feb. 1, 2017 to Jan. 31, 2018 with umbilical cord clamping after umbilical artery stopped beating). The first day total serum bilirubin level, transcutaneous bilirubin (TcB) level at 1-3 d after birth, the starting time of blue light therapy, the blood routine at 1 d and 7 d after birth and the incidence of intracranial hemorrhage were compared between the two groups. Results A total of 308 preterm infants were included in this study, with 165 cases in the DCC group and 143 cases in the ECC group. There were no significant differences in the basic information of maternal and premature infants, the first day total serum bilirubin level, the starting time of blue light therapy, white blood cell count, platelet count, or the incidence of intracranial hemorrhage between the two groups (all P>0.05). The TcB levels on the 2^nd and 3^rd d after birth and the hemoglobin levels and hematocrit on the 1^st and 7^th days after birth in the DCC group were significantly higher than those in the ECC group (all P<0.05). Conclusion DCC can increase hemoglobin level and hematocrit in the early stage of moderate and late preterm infants. It can increase TcB level to a certain extent, but blue light therapy is not necessary for early intervention. DCC does not increase the incidence of intracranial hemorrhage, making it safe and effective for moderate and late preterm infants.