This paper summarizes the infection control practice in coronavirus disease 2019 (COVID-19) wards of Wuhan Huoshenshan Hospital. By closely focusing on the three key elements of infectious diseases and strictly following the general prevention principles, we implement systematic management, including ward design, personnel management, disinfection measures, protection management, diagnosis and treatment path, clinical specimens, redisinfection of medical equipment and clearance of garbage. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid was detected in samples from critical patients and their surrounding environments, so as to indentify the possible contamination path to guide the prevention and control. During the mission, the systematic and meticulous infection control management in the wards effectively controlled cross infections, ensured the effective and safe treatment of COVID-19 patients, with no medical staff infections occurred, providing references for infection control of wards in similar epidemics in the future.