Objective To investigate the effect of electret electrostatic field on migration of macrophages. Methods The －2 000 V polypropylene electret was prepared by low temperature plasma discharge (grid controlled constant voltage corona discharge) at room temperature. The charge storage stability of the electret under storage and experimental conditions was studied by conventional isothermal surface potential decay measurement. The mouse macrophages (RAW264.7 cells) in logarithmic growth phase were treated by ultraviolet sterilized electret. The cell migration and morphological changes under the electret electrostatic field were analyzed by cell scratch test, Transwell assay and fluorescent probe tracing. Results The surface potential of －2 000 V electret was stable at 70% of its initial value when it was placed at room temperature and normal humidity for 14 d. There was no significant difference between the surface potential of －2 000 V electret in cell incubator for 28 h and that for 0 h (P>0.05). The results of the cell scratch test showed that after exposed to the electret electrostatic field for 28 h, the migration and proliferation ability of RAW264.7 cells to scratch area was enhanced, and the scratch area decreased. The results of the Transwell assay showed that the number of transmembrane cells in electret treated group increased compared with the control group at 12 and 24 h (both P<0.01). The results of the fluorescent probe tracing showed that RAW264.7 cells became larger, expanded, with increased ruffling, and produced protuberant structures such as plate-shaped pseudopodia and filamentous pseudopodia under the action of negative electret. Conclusion The prepared －2 000 V electret can provide a stable electrostatic field in vitro and continuously act on cells. The electrostatic field can promote the macrophages to become larger, extended, with increased ruffling, and produce protuberant structures such as plate-shaped pseudopodia and filamentous pseudopodia, which can improve the migration ability of macrophages.