Objective To analyze the clinical pregnancy status of embryos derived from non-pronucleus (0PN) zygotes and one pronucleus (1PN) zygotes in short-term in vitro fertilization (IVF) fresh transplant cycles, and to explore the clinical value of these embryos. Methods The laboratory data of 524 fresh transplant cycles of 524 infertile patients who received short-term IVF-embryo transfer treatment at Department of Reproductive Medicine, The First Affiliated Hospital of Naval Medical University (Second Military Medical University) from Jan. 2016 to Jan. 2020 were retrospectively analyzed. The transfer cycles were divided into group A and group B according to the embryo source. The embryos in group A were derived from 0PN or 1PN zygotes, and those in group B were derived from two pronuclei (2PN) zygotes. According to the number of embryos transferred, the single-embryo transferred ones were divided into group A1 (with embryos derived from 0PN or 1PN zygotes) and group B1 (with embryos derived from 2PN zygotes); and the double-embryo transferred ones were divided into group A2 (with embryos derived from 0PN＋0PN zygotes, 1PN＋1PN zygotes, or 1PN＋0PN zygotes) and group B2 (with embryos derived from 2PN＋2PN zygotes). Clinical and laboratory data and pregnancy outcomes were compared between groups A and B, A1 and B1, and A2 and B2, respectively. Results The numbers of eggs obtained per cycle in group B and group B2 were significantly greater than those in group A and group A2, respectively (both P＜0.01), while there was no significant difference between group A1 and group B1 (P＞0.05). There were no significant differences in implant rate, clinical pregnancy rate, abortion rate or live birth rate between group A and group B, group A1 and group B1, or group A2 and group B2 (all P＞0.05). Conclusion In the short-term IVF fresh transfer cycle, the cycle with more number of eggs obtained per cycle is more likely from embryos derived from 2PN zygotes. There is no significant difference in pregnancy outcome between embryos derived from 0PN/1PN zygotes and 2PN zygotes in short-term IVF fresh transfer cycle. Embryos derived from 0PN/1PN zygotes can be transferred with informed consent of the patient.