Objective To explore the feasibility of mobile blood bank in blood support for large surface warship and the operation mode of emergency blood collection and supply through emergency blood collection and supply drill. Methods The emergency blood collection and supply drill was developed in advance, and the feasibility of the plan was tested by organizing practical exercises during offshore training. At the same time, the time period and existing problems of each step in the drill were recorded. Results It took about 35 min from the time when the blood collection personnel was notified by the radio to the time when he/she completed the blood type identification, cross matching and whole blood collection. The blood collection personnel had proven skills, and no adverse reactions occurred in the blood donors. Conclusion The simulation results show that mobile blood bank can be used as a major way of blood support for large surface warships, but it still needs further optimization.