Objective To establish a minimally invasive transverse aortic constriction (TAC) technique in mice without endotracheal intubation and ventilator ventilation. Methods Forty male C57BL/6 mice aged 2-4 months were randomly divided into TAC-operated group and sham-operated group, with 20 mice in each group. The mice in the TAC-operated group were anesthetized by intraperitoneal injection of sodium pentobarbital. After partial sternotomy, the aortic arch was exposed, and a suture was passed under the aortic arch and the aortic arch was tied to a blunt 90° curved 27-gauge needle, and then the needle was pulled out. The operation procedure of the sham-operated group was the same as that of the TAC-operated group, but no ligation was performed. The heart weight/body weight ratio, gene expression of cardiac hypertrophy markers and in vivo cardiac function of the 2 groups were compared and analyzed. Results Compared with the sham-operated group, the heart weight/body weight ratio of the mice was significantly increased on the 28^th day after operation ([6.21±0.20]mg/g vs[3.94±0.19]mg/g, P<0.01), the expression of myocardial hypertrophy marker genes such as atrial natriuretic peptide and brain natriuretic peptide was significantly increased (3.48±0.37 vs 1.00±0.12, 3.92±0.41 vs 1.00±0.15, both P<0.01), and both left ventricular ejection fraction and left ventricular fraction shortening were significantly decreased in the TAC-operated group ([40.18±1.96]% vs[53.26±2.32]%,[23.12±2.08]% vs[32.16±2.64]%, both P<0.01). Furthermore, the survival rate was 95.00% (38/40) during surgery and within 28 d after surgery. Conclusion Minimally invasive TAC surgery can effectively induce myocardial hypertrophy and decrease cardiac function in mice, with low mortality.