Chronic pancreatitis is an irreversible chronic pancreatic fibrosis disease. Pancreatic stellate cells are the key cells of fibrosis, and activated ones can secrete extracellular matrix to promote the development and progression. Pancreatic acinar cell necrosis and macrophage infiltration are crucial in the process of pancreatic fibrosis. After necrosis, acinar cells would release various substances to activate pancreatic stellate cells, and that may be the initiating factor of fibrosis. The polarization and phenotypic switch of macrophages would affect the regeneration and repair process of pancreatic tissue. The interactions among pancreatic stellate cells, acinar cells and macrophages can jointly regulate the process of pancreatic fibrosis. This article reviews the latest progress on the roles of the above 3 kinds of cells in pancreatic fibrosis of chronic pancreatitis.