Objective To analyze the risk factors of non-infectious fever after uterine myomectomy. Methods The clinical data of 146 patients who underwent myomectomy in our hospital from Jan. 2021 to Jan. 2022 were retrospectively analyzed. Postoperative non-infectious fever refers to the body temperature≥38 ℃ within 48 h after operation, and there is no evidence of infection. Univariate analysis was used to analyze the influence of age, preoperative and postoperative hemoglobin concentration, perioperative antibiotic use, maximum size of myomas, number of myomas, suture mode, operation mode, whether to penetrate the endometrium during operation, whether to perform uterine cavity operation at the same time, operation time and other factors on postoperative non-infectious fever. Multivariate binary logistic regression analysis was performed on the selected suspected risk factors. Results Among the 146 patients, 25 had postoperative non-infectious fever and 121 had normal body temperature. Univariate analysis showed that operation time, operation mode, suture mode, number of myomas, maximum size of myomas, history of cesarean section, preoperative and postoperative hemoglobin concentration, preoperative and postoperative hemoglobin reduction and postoperative hospital stay would affect the postoperative non-infectious fever (all P＜0.05). The results of multivariate logistic regression analysis showed that the operation time (odds ratio ［OR］ ＝1.01, 95% confidence interval (CI) 1.00-1.02, P＝0.03), operation mode (OR＝5.03, 95% CI 1.74-14.56, P＜0.01) and the number of myomas (OR＝1.33, 95% CI 1.05-1.68, P＝0.02) were independent risk factors for the occurrence of postoperative non-infectious fever (all P＜0.05). Conclusion The independent risk factors of non-infectious fever after myomectomy include operation time, operation mode and number of myomas. These factors should be intervened in clinical work.