The combination of single-cell transcriptome sequencing and spatial transcriptome sequencing can analyze the transcriptome and spatial location information of each cell within the tissue, which helps to explore the gene expression level of cells and the 3-dimensional reconstruction of tissue, decipher cell heterogeneity, understand the connections and interactions between cells, and deeply explore the cellular and molecular mechanisms of disease development and progression. Fibrotic diseases involve multiple organs, the course of the disease is extremely complex, and there is a lack of effective anti-fibrotic treatment; it is still necessary to strengthen the comprehensive understanding of the disease. In recent years, single-cell transcriptome sequencing combined with spatial transcriptome sequencing has been applied for the research on pathophysiological mechanisms of fibrotic diseases, and more progress has been made. This article briefly introduces single-cell transcriptome sequencing combined with spatial transcriptome sequencing and reviews its research progress in fibrotic diseases.