Objective To establish a multidisciplinary team (MDT)-based management program for the prevention and control of multi-drug resistant organism (MDRO) infection in cerebrovascular intensive care unit (ICU) and to evaluate its efficacy. Methods A total of 161 patients with cerebrovascular diseases, who met the admission criteria and were admitted to the ICU of Department of Cerebrovascular Medicine of The First Affiliated Hospital of Naval Medical University (Second Military Medical University), were enrolled using convenience sampling method. Among them, 79 patients (admitted from Jan. to Jun. 2022) were in control group and 82 patients (admitted from Sep. 2022 to Feb. 2023) were in intervention group. The control group received routine infection control management, while the intervention group received the MDRO infection prevention and control management program based on MDT in ICU of cerebrovascular medicine departments. The incidence of MDRO infection of the patients and the compliance rates of MDRO prevention and control measures (including the implementation of isolation measures, isolation medical orders, isolation identification and disinfection measures of the patients, and the hand hygiene implementation and awareness of MDRO prevention and control knowledge of medical staff) were analyzed and compared between the 2 groups. Results In the intervention group, the incidence of MDRO infection was significantly decreased from 45.57% (36/79) in the control group to 28.05% (23/82) (P＜0.05); the compliance rates of implementation of isolation measures, isolation medical orders, isolation identification and disinfection measures of the patients, and hand hygiene implementation and awareness of MDRO prevention and control knowledge of medical staff were significantly increased from 79.63% (86/108), 70.51% (55/78), 76.47% (78/102), 71.32% (97/136), 86.28% (283/328), and 82.24% (125/152) to 92.00% (69/75), 90.00% (63/70), 92.50% (74/80), 93.48% (86/92), 92.66% (265/286), and 93.91% (108/115), respectively (all P＜0.05). Conclusion MDT-based MDRO infection prevention and control management program can effectively reduce MDRO infection in ICU of cerebrovascular medicine departments and improve the compliance of MDRO nosocomial infection prevention and control.