Objective To construct an on-the-job training program for brain and heart health managers, so as to provide reference for their training. Methods According to the documents on the construction and development of stroke centers and the responsibilities of brain and heart health managers issued by the Stroke Prevention and Treatment Engineering Committee of National Health Commission of China, a preliminary draft of the training program for brain and heart health managers was constructed by literature review and group discussion, considering the training status of brain and heart health managers in China. Delphi method was used to consult 15 experts from 9 tertiary first-class hospitals in 6 provinces on stroke treatment, nursing, management, and education to further form an on-the-job training program for brain and heart health managers. Results A total of 2 rounds of expert consultation were conducted. The effective response rates of the 2 rounds of expert consultation questionnaires were 100%, and the expert authority coefficients were 0.913 and 0.917, respectively. The final on-the-job training program for brain and heart health managers was formed, including training conditions of access (9 items), training objectives (5 items), training content (3 level-1 items, 8 level-2 items, and 39 level-3 items), and training methods and evaluation (4 level-1 items and 10 level-2 items). Conclusion The on-the-job training program for brain and heart health managers in this study is scientific and reliable, and can provide guidance and reference for standardized job training for brain and heart health managers; however, it needs further improvement.