Objective To observe the changes of plasma gastrointestinal hormones in motion sickness sensitive and insensitive individuals before and after vertical oscillation stimulation, and to construct a susceptibility prediction model for motion sickness. Methods A total of 60 healthy male volunteers were enrolled to receive sinusoidal vertical oscillation stimulation for 45 min. The motion sickness susceptibility questionnaire (MSSQ) was filled out before the experiment. Immediately after motion, the severity of motion sickness was evaluated by Graybiel scale. The motion sickness sensitive (Graybiel score≥8 and MSSQ susceptibility index＞21, n＝15) and insensitive (Graybiel score≤2 and MSSQ susceptibility index＜5, n＝15) participants were screened. Plasma levels of glucagon-like peptide-1 (GLP-1), cholecystokinin (CCK), leptin, ghrelin, neuropeptide Y (NPY) and orexin A (OXA) were detected by enzyme-linked immunosorbent assay before and after vertical oscillation stimulation. Logistic regression model was used to analyze the predictive effect of plasma gastrointestinal hormone levels on susceptibility to motion sickness, and a combined predictive model was established. Receiver operating characteristic (ROC) curve was used to analyze predictive value of the model. Results Ghrelin and CCK levels were significantly increased in the sensitive group after stimulation compared with those before stimulation (both P＜0.01), while NPY and leptin levels were significantly decreased (both P＜0.01). Similar results were also observed when compared with the insensitive group after stimulation. Multivariate logistic regression analysis showed that plasma ghrelin, CCK and NPY were independent predictors of susceptibility to motion sickness. The established susceptibility prediction model for motion sickness was logit(P)＝－0.051×ghrelin＋0.060×NPY－0.169×CCK＋33.397. ROC curve analysis showed that area under curve (AUC) value of the prediction model was 0.988, the sensitivity and specificity were 100.0% and 93.3%, respectively, and the prediction effect was better than ghrelin, CCK and NPY alone (AUC＝0.792, 0.880, 0.838). Conclusion The changes of peripheral gastrointestinal appetite regulating hormone levels may be related to the susceptibility to motion sickness. The combined use of these indicators can predict the susceptibility to motion sickness.