Objective To establish an inducible knockout mouse model of trafficking protein particle complex subunit 11 (Trappc11). Methods and results LoxP sites were introduced on both sides of exon 3-5 of Trappc11, and then the CRISPR/Cas9 technique was used to establish F0 C57BL/6J mice. The positive F0 generation mice were identified by polymerase chain reaction amplification and sequencing. After that, F0 positive mice were mated with C57BL/6J wild type mice to obtain F1 Trappc11^flox/+ mice. And then, Trappc11^flox/+ mice were mated with UBC-CreERT2 mice, and finally Trappc11 inducible systemic knockout mouse model was obtained after 2 generations. Conclusion The Trappc11 inducible knockout mouse model is established using CRISPR/Cas9 and Cre-loxP, providing an important tool for revealing the pathophysiological role of Trappc11 in multi-organ system diseases.