Objective To develop a full system to monitor G-induced loss of consciousness (G-LOC) of pilots caused by continuous positive acceleration and wake up them in time, so as to shorten the airborne unconsciousness time of pilots. Methods The system used MPU6050 sensor and SICHIRAY muscle sensor to obtain the head posture and electromyogram (EMG) characteristics of the pilot’s environment in real time. After system hardware design, main program design, head posture monitoring module design, EMG signal acquisition and processing module and upper computer interface display design, the system test and centrifuge test were carried out. The system test set the threshold of the EMG signal and the head posture angle by collecting the EMG signals of the subjects 2 min at rest and under muscle contraction and the head posture angle of the human body looking forward and bowing as hard as possible, and the acceleration threshold was the gravity acceleration in the test environment. The centrifuge test simulated the process of raising and bowing of the human body by placing the position of the attitude angle on the animal centrifuge. The acceleration threshold was set at 3 G, and the positive acceleration of this test environment was 4, 6, and 8 G. Results The system could capture the head posture and the physiological characteristics of EMG signals in real time, send out voice reminders in time, and could immediately enter the alarm wake-up mode when failed to receive feedback (head up, EMG recovery) within fixed time. And it could be used on centrifuges. Conclusion This system has complete function, complete logic and strong practicability. It is possible for practical application after further experimental improvement.