Objective: To investigate the growth and the immunogenicity-related phenotype of B cell-human hepatoma cell fusion cells after 60Co-radiation. Methods: Growth curve and soft agar colonogenicity of fusion tumor vaccine in vitro, and their tumorigenicity in nude mice in vivo for the radiated cell fusion tumor vaccine were observed. The expression of MHC-Ⅰ,MHC-Ⅱ, B7 and hepatoma-antigen(GP75) that were related to immunogenicity of the radiated fusion tumor vaccine cell were analyzed by flow cytometry. Results: 60Co-radiated fusion cells lost proliferative capacity, partly died on d 4 and almost completely died on d 7,while MHC-Ⅰ,MHC-Ⅱ, B7 and GP75 had no apparent changes in immunogenicity-related phenotype in the first 3 d. Conclusion: 60Co-radiated fusion cells lose proliferative capacity, but they have no apparent changes in immunogenicity-related phenotype in the first 3 d.