Objective: To study the expression of integrins in fibroblasts in patients with scleroderma. Methods: Employing technique of indirect immunocolloidal gold and electron microscope, we observed the expression of integrin α5 ,β1 subunits and the supermicro-structures of sclerderma fibroblasts cultured in medium absorbed with fibronectin. Results: The results showed that the expression of integrin α5 ,β1 subunits in fibroblasts from scleroderma was higher than that in normal fibroblasts (P<0.01), and colloidal golds clustered on the surfaces of scleroderma fibroblasts. The cytoskeletal structure: microfilaments were dense and aggregated into bundles. Conclusion: All of these results suggest that the interactions of fibroblasts with fibronectin mediated by integrin α5 ,β1 subunits increase in scleroderma