Objective: To enhance the efficiency of bone marrow transplantation (BMT) and to provide a simple and effective method for cryopreservation of bone marrow cells (BMCs). Methods: BMCs were cryopreservated at -80℃ and a new method for bone marrow scheduled transplantation (BMST) was studied with acute radiation disease model of Kunming mice. Results: The method for cryopreservation of BMCs at -80℃ produced the highest recovery rates for nucleated cells (89.0%±3.5%), the trypan blue exclusion (85.0%±3.6%), CFU-GM ［(108±10)/105 BMC］, CFU-S［(12±3)/105 BMC］. Using this method, lethally irradiated Kunming mice received allogenetic BMST. The survival rate of mice (30%) in BMST group transplanted with 4×105 BMCs was the same as that in once BMT group transplanted with 1×107 BMCs. And the survival rate of mice (60%) in BMST transplanted with 4×106 BMCs was significantly higher than that in once BMT. Evidence of engraftment could be detected in all groups. Conclusion: This method for cryopreservation BMCs at -80℃ is simple and effective, and these data suggest that “Niches” vacated each time could be fully used in BMST, so the transplanted BMCs could be decreased, the engraftment of stem cells could be increased, and the grafting efficiency could be enhanced. This method is a new method aiming at “both seed and soil”.