Objective: To investigate the effect of reserpine and verapamil on Neisseria gonorrhoeae′s multiple antibiotic resistance. Methods: Rapid quantitative method of minimum inhibitory concentration(MIC) was used for susceptibility testing on 30 strains of N. gonorrhoeae, including standard strain of WHO, A,B,C,D,E strains and strains isolated from outpatients. The influences of reserpine and verapamil on N. gonorrhoeae′s multidrug resistance were tested after they were added to 6 antimicrobials, respectively. Results: A specific concentration of reserpine could reduce MIC of antimicrobials on N.gonorrhoeae strains by 86%,while verapamil by 81%. Conclusion: Reserpine and verapamil have reverse effect on N. gonorrhoeae′s multiple antibiotic resistance, both of them can reduce the antimicrobials′ MIC to N. gonorrhoeae.