Objective：To study the expression of P16 gene and its correlation with clinical cases. Methods：The levels of P16 gene products were examined immunochemically in resected specimens of human laryngeal squamous cell carcinoma. Results： Carcinomas tested： 47.62%（20/42） showed positive staining in the lesions. 40.00%(4/10) in supraglottic cancer group, 57.89%(11/19) in glottic cancer group, 38.46%(5/13) in subglottic cancer group, 75.00%(12/16) in cell differentiation gradeⅠgroup, 30.77%(8/26) in cell differentiation grade Ⅱ？Ⅲ group, 20.00%(3/15) in lymph node metastasis group, 62.96%(17/27) in those without metastasis, 40.00%(8/20) in clinical stageⅠgroup, 54.54%(12/22) in stageⅡ？Ⅳgroup. Conclusion：The expression of P16 protein is related with malignant degree and lymph metastasis in human laryngeal carcinoma.