Objective: To provide a permanent wound coverage by using skin allografts in mouse model with fullthickness burn of 20%TBSA. Methods: The recipient mouse was treated with 9 Gy total body irradiation from 60Co source, followed by infusion of a mixed donor and recipient T-cell depleted bone marrow where a state of specific unresponsiveness to the skin allograft was induced without the need for chronic immunosuppression. Five experimental groups were divided: group Ⅰ, 20%TBSA burn of C57B/6 mice with skin allograft from a BALB/c mouse at 48 h without irradiation; group Ⅱ, similar to group Ⅰ but with irradiation; group Ⅲ, similar to group Ⅱ but with the infusion of mixed bone marrow; group Ⅳ, similar to group Ⅲ but with skin allograft from a C3H/He mouse; group Ⅴ, controlled C57BL/6 mice with homograft. Results: There were no statistical difference irranimal mortality in all groups except group Ⅱ, and no evidence of infectious morbidity and graft vs host disease were found. Mean skin allograft survival (mice died with intact graft were excluding) was as follows: group Ⅰ, (8.4±1.5) d; group Ⅱ, (17.5±3.7) d; group Ⅳ, (32.5±8.1) d, and group Ⅴ, 90 d; gorup Ⅲ, (78.2±5.6) d (P<0.05 vs group Ⅰ, Ⅱ and Ⅳ). Conclusion: C57BL/c mice exhibited specific transplantation unresponsiveness to the BALB/c mouse skin, but rejected the third party MHC-disparate C3H/He mouse skin. This study suggests the potential use of induced specific unresponsiveness to skin allografts for wound coverage in thermal injury.