Objective: To study the mechanism of Traditional Chinese Medicine Tuojiagao in cleaning necrotic and denatured tissue in deep burn wound. Methods: Two groups of patients with deep burn wound were studied: (1) Traditional Chinese Medicine Tuojiagao group, (2) Ag-SD frost group. Tissue and exudate of the wound were collected. Macrophages were marked with anti-CD68 antibody in tissue slides. The bacteria of wound exudate were counted. Results: The sections from wounds after using Tuojiagao have more CD68+ cells (macrophages) than Ag-SD group, and no difference of bacteria count was found between the 2 groups. Conclusion: Tuojiagao could clean necrotic tissue through recruiting macrophages to scavenge and break down it.