Objective: To demonstrate whether some important inflammatory markers rise in degenerative cervical intervertebral disc, and to investigate pathogenesis of cervical spondylosis in biochemistry. Methods: In this experiment,20 white New Zealand rabbits were used and divided into 2 groups: control group and model group. Each group consisted of 2 subgroup: 3 month group and 8 month group. The experimental model of degenerative cervical disc in the rabbit was established by resection of the cervical supraspinous and interspinous ligaments and detachment of the posterior paravertebral muscles from the cervical vertebrae. Then animals in each group were killed 3 months and 8 months after operation, respectively. The concentrations of histamine,5-OH-tryptamine,PGE2 and 6-keto-PGF1α were assayed in all animal disc samples. Results: The contents of 4 inflammatory mediators from the discs of model groups were overtly higher than that of control groups, respectively. The contents of 4 inflammatory mediators had no statistical difference between model groups of 3 month and 8 month. Conclusion: The degenerated cervical disc is inflammatory, and the inflammatory mediators produced by the degenerated cervical intervertebral disc may be directly related to the pathogenesis of cervical spondylosis