Objective: To amplify large number of dendritic cells (DCs) from murine bone marrow and assay their morphological, immunological characteristics. Methods: Lymphocytes, monocytes-macrophages and granulocytes of murine bone marrow were depleted using immuno-Minimagnetic cell sorter. The selected cells were cultured with granulocyte-macrophage colony-stimulating factor, and the floating cells were collected 8 d after culture. The phenotype of collected DCs was analyzed, and their morphology was observed under electron microscopy. Results: (2-5)×106 DCs were obtained from bone marrow of one mice, and the purity of DCs was more than 80%. The cells expressed specific antigens (DEC205) of DCs and had typical morphology of DCs. Conclusion: A method to generate large number of DCs from murine bone marrow is established, which contribute to further studying immunological functions of DCs.