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| 三种人工合成可降解血管支架材料体外生物相容性及表面改性研究 |
| 杨松林,谷涌泉,汪忠镐,张建,陈兵,王连才,姜明,郑江红,刘庆阳,YANGSong-lin,GUYong-quan,WANGZhong-gao,ZHANGJian,CHENBing,WANGLian-cai,JIANGMing,ZHENGJiang-hong,LIUQing-yang |
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| 摘要: |
| 目的:对聚对苯二甲酸丁二醇酯-co-聚对苯二甲酸环己烷二甲醇酯-b-聚乙二醇嵌段共聚物(T20)、聚3羟基丁酸-3-羟基戊酸酯(PHBV)和聚对苯二甲酸丁二醇酯/聚乙二醇对苯二甲酸酯嵌段共聚物(PEGT/PBT)共三种人工合成的可降解血管支架材料进行体外生物相容性评价,并观察它们表面改性后对犬血管平滑肌细胞黏附生长的影响.方法:通过测定接触角观察三种支架材料的亲水性;MTT法、流式细胞术(FITC)及扫描电镜观察犬血管平滑肌细胞在T20、PHBV和PEGT/PBT三种支架上的增殖及生长情况,评价它们的体外生物相容性;采用明胶及多聚赖氨酸对三者进行表面预处理,MTT法比较三者表面改性后犬血管平滑肌细胞的黏附生长情况.结果:三种材料的接触角均小于90°,亲水性的大小顺序为:PEGT/PBT＞T20＞PHBV.MTT法显示犬血管平滑肌细胞可以在PHBV、PEGT/PBT、F20支架材料上黏附生长,接种24 h后细胞活性升高(PEGT/PBT＞T20＞PHBV,P＜0.01),随后降低,72 h后又开始增高(T20＞PEGT/PBT＞PHBV,P＜0.01);FITC荧光染色可见犬血管平滑肌细胞在载体上成片生长,状态良好;扫描电镜见犬血管平滑肌细胞在载体上呈片状融合生长,并见大量的细胞外基质.MTT法结果表明三种材料经过明胶或多聚赖氨酸表面改性预处理后细胞增殖活性明显高于未处理组(P＜0.01).结论:T20、PHBV、PEGT/PBT血管支架材料具有良好的体外生物相容性,对其进行表面改性后可明显提高犬血管平滑肌细胞的黏附生长能力. |
| 关键词: 支架、血管、生物相容性、表面改性 |
| DOI：10.3724/SP.J.1008.2006.00400 |
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| 基金项目:北京市科委计划基金资助（H020920040330）. |
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| In vitro evaluation of biocompatibility and surface modification of 3 kinds of artificial graft material |
| 杨松林,谷涌泉,汪忠镐,张建,陈兵,王连才,姜明,郑江红,刘庆阳,YANG Song-lin,GU Yong-quan,WANG Zhong-gao,ZHANG Jian,CHEN Bing,WANG Lian-cai,JIANG Ming,ZHENG Jiang-hong,LIU Qing-yang |
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| Abstract: |
| Objective：To evaluate the biocompatibility of 3 kinds of artificial,biodegradable graft materials, including T20 [Poly （ butylene terephthalate）-co-poly （ cyclohexylene dimethanol terephthalate）-b-poly （ ethylene glycol）], PHBV （ Poly3hydroxybutyrate-Poly3-hydroxyvalerate） and PEGT/PBT [Poly （butylene terephthalate）/poly （ethylene glycol） terephthalate], and to study their effects on canine smooth muscle cells（SMCs） growth after surface modification. Methods： The contact angles were measured to compare the hydrophilicity of T20, PHBV, and PEGT/PBT scaffolds. Then SMCs were cultivated on these biodegradable scaffolds; their proliferation and growth were assayed by MTT assay, FITC and electron microscopic observation for evaluation of their in vitro biocompatibility. The growth of SMCs was also assayed on the 3 scaffolds after they were pretreated with gelatin or Poly-l-lysine. Results： The contact angles of 3 scaffolds were all less than 90°（PEGT/PBT〉T20〉PHBV）. MTT assay showed that SMCs adhered to and grew well on T20, PHBV, and PEGT/PBT scaffolds. The cell viability began to increase 24 h after cultivation （PEGT/PBT〉T20〉PHBV, P〈0.01）, followed by a decrease, but increased again 72 h after cultivation （T20〉PEGT/PBT〉PHBV, P〈0.01）. FITC observation showed that SMCs grew well and electron microscopic observation showed a confluent growth of SMCs with abundant extracellular matrix. The viability of SMCs was significantly higher on scaffolds pretreated with gelatin or Poly-l-lysine than on those without pretreatment（P〈0.01）. Conclusion： T20,PHBV,PEGT/PBT have good in vitro biocompatibility; the scaffolds made of these materials can obviously improve cell attachment after surface modification. |
| Key words: stents blood vessels biocompatibility surface modification |
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