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移植肾急性排斥时Fas配体和T细胞内抗原1的表达 |
张万君,梅长林,叶朝阳,赵学智,孙田美,ZHANGWan-jun,MEIChang-lin,YEChao-yang,ZHAOXue-zhi,SUNTian-mei |
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摘要: |
目的:探讨移植肾发生急性排斥反应时肾组织Fas配体(Fas ligand,FasL)和T细胞内抗原1(T-cell intracellular antigen-1,TIA-1)的表达及意义.方法:应用免疫组织化学技术分别检测32例移植肾标本、2例供肾标本及8例肾癌周围肾组织标本FasL和TIA 1的表达,标本按Banff标准分为急性排斥(14例)、慢性排斥(15例)和无排斥改变(13例)3组,分析移植肾组织标本病理形态学改变与FasL和TIA 1表达的相关性.结果:FasL主要在肾小管上皮细胞胞质内表达,急性排斥组表达明显强于慢性排斥组和无排斥组(P<0.05);TIA-1在肾小管上皮细胞和间质浸润淋巴细胞均有表达,小动脉内皮细胞亦可见阳性着色,急性排斥组阳性强度较高,与慢性排斥组和无排斥组间存在显著差异(P<0.05);急性排斥标本FasL和TIA-1的表达强度与组织损害的严重程度呈正相关.结论:急性排斥时移植肾组织FasL和TIA-1表达增强是T细胞活化的反映,其检测可能有助于移植肾急性排斥的早期诊断. |
关键词: 肾移植、移植物排斥、Fas配体、T细胞内抗原1 |
DOI:10.3724/SP.J.1008.2006.00409 |
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基金项目: |
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Expression of Fas ligand and T cell intracellular antigen-1 during acute rejection after renal transplantation |
张万君,梅长林,叶朝阳,赵学智,孙田美,ZHANG Wan-jun,MEI Chang-lin,YE Chao-yang,ZHAO Xue-zhi,SUN Tian-mei |
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Abstract: |
Objective:To study the intrarenal expression of Fas ligand (FasL) and T cell intracellular antigen-1 (TIA-1) protein during acute rejection after renal transplantation. Methods: Immunohistochemical technique was used to detect FasL and TIA-1 expression in 32 transplanted renal samples,2 living donor kidneys and 8 adjacent samples of renal cancer (including 14 acute rejection samples, 15 chronic and 13 non-rejection ones according to Banff criteria). Results: The positive FasL staining was observed mainly in cytoplama of renal tubule epithelia,and the positive rate was significantly higher in acute rejection samples than in others(P〈0.01 ). TIA-1 was found in both tubular epithelial cells and interstitial infiltrating lymphocytes,occasionaly in small arterial endothelial cells. The positive staining rate of TIA-1 acute rejection samples was also higher than those of other samples(P〈0.05). The expression of FasL and TIA-1 in acute rejection samples was positively correlated with the severity of tissue damages. Conclusion: Over expression of FasL and TIA-1 in acute rejection renal allograft tissues may indicate the activation of T cells,and its detection may be helpful for early diagnosis of acute rejection |
Key words: kidney transplantation graft rejection Fas ligand T-cell intracellular antigen-1 |