| 摘要: |
| 目的:对<中国药典>2005版一部中知母药材的菝葜皂苷元含量测定的前处理条件进行改进.方法:采用L9(33)正交设计表,对超声时间、提取溶剂乙醇的比例以及超声次数等因素进行优化.为确保色谱方法的沿用性,还做了加样回收率考察.结果:最终确立了70﹪乙醇超声20 min提取2次作为最优的提取条件,再进行水解得到皂苷元进行含量测定.结论:与药典方法比较,改进后的方法测定的含量结果有了较大提高,能够反映出样品中真实的菝葜皂苷元含量. |
| 关键词: 知母、菝葜皂苷元、提取法 |
| DOI：10.3724/SP.J.1008.2006.00790 |
| 投稿时间：2006-04-21修订日期：2006-05-25 |
| 基金项目:上海市科委重大项目(2403DZ19548);上海市科委科技攻关项目(02419125). |
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| Pretreatment of sarsasapogenin in Rhizoma Anemarrhenae: a research of methodology |
| ZHU Dong-liang,LI Xiang,LOU Zi-yang,DONG Xin,CHAI Yi-feng |
| (第二军医大学药学院药物分析学教研室,上海,200433) |
| Abstract: |
| Objective：To improve the pretreatment method described in ChP （2005 Edition, Vol I ） for the determination of sarsasapogenin in Rhizoma Anemarrhenae. Methods： According to orthogonal design L9 （33）, the extraction time, alcohol concentration, extraction frequency,etc, were modified to achieve the best extraction outcome with ultrasonic wave extraction. Results： The optimal extraction condition included 70%0 alcohol and twice ultrasonic wave extraction,20 min each time. Finally hydrolization was applied to obtain sarsasapogenin for determination. Conclusion： Compared with the pretreatment method in ChP, this improved extraction method shows a higher sarsasapogenin content in determination and is more accurate in reflecting the true content of sarsasapogenin in Rhizoma Anemarrhene |
| Key words: Rhizoma Anemarrhene sarsasapogenin extraction |
