Objective： To construct a recombinant retroviral vector expressing pancreatic duodenal homeobox-1 （PDX-1） gene and express the PDX-1 expression cassette in liver progenitor cells （LEPCs）, so as to study the conversion of hepatic cells to pancreatic-like cells, Methods： The full length of PDX-1 gene was amplified by PCR and subcloned into pMSCVpuro vector. The pMSCV PDX-1 puro was then introduced into Phoenix package cells and the cell culture supernatant was used for Ping- Pong infection of another packaging cell line PT67 to obtain stable virus-producing cell line. Results： The pMSCV PDX-1 puro vector was successfully constructed and transfected into PT67 cells. The viral supernatants of PT67 cells efficiently infected LEPCs in vitro and the infected LEPCs stably expressed PDX-1 gene, Conclusion： The pMSCV PDX-1 puro vector and PDX-1 expressing LEPCs have been successfully constructed, laying a basis for studying the transdifferentiation of liver progenitor cells to pancreas lineage cells.