Objective： To study the MR spectroscopic characteristics of orbital tumors and normal tissues in vitro by the super high field MR, so as to provide theoretical basis for clinical application of MR spectroscopy （MRS）. Methods： Ten tumor specimens （including 7 benign ones and 3 malignant ones） and 2 normal tissue samples received MR spectroscopic examination by BRUKER AVANCE 400（SB）super high field MR, and their MR spectroscopic characteristics were compared. Results： ^1H- MRS with super high field MR showed that the Cho and Cr peaks of malignant orbital tumors were higher than those of benign tumors. Three consecutive single peaks were noticed around 2 × 10^-6 in the MRS of benign and malignant tumors, but not in that of normal tissues. Conclusion： ^1H-MRS can differentiate benign, malignant orbital tumors and normal tissues, making it worth of further study for clinical application.