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| 贻贝水溶性多糖MP-Ⅰ的分离纯化及体外抗肿瘤活性研究 |
| 徐红丽,郭婷婷,郭一峰,张建鹏,冯伟华,焦炳华 |
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| (第二军医大学基础医学部生物化学与分子生物学教研室,上海,200433;上海水产大学生命学院中心实验室,上海,200090) |
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| 摘要: |
| 目的:分离纯化贻贝多糖MP-Ⅰ,并进行体外抗肿瘤活性的研究.方法:采用热水提取,Sevage法除蛋白分离得粗多糖,DEAE-Sepharose、Sepharose CL-6B柱层析纯化的方法从东海厚壳贻贝中得到贻贝多糖纯品MP-Ⅰ.用GC及TLC法进行单糖组分的分析,用MTT法进行体外抗肿瘤活性的研究.结果:得到贻贝多糖纯品MP-Ⅰ,多糖的得率为2.14﹪.单糖组分分析显示MP-Ⅰ主要由葡萄糖组成,MP-Ⅰ(0.5,0.1,0.02 mg/ml)对体外HO-8910、MCF-7、K562、SMMC-7721肿瘤细胞均有不同程度抑制作用(P＜0.01),其中高浓度组对HO-8910、MCF-7肿瘤细胞的抑制率分别达到30.55﹪和36.38﹪.结论:贻贝多糖MP-Ⅰ主要由葡萄糖组成,对HO-8910、MCF-7、K562、SMMC-7721肿瘤细胞有体外抑制作用. |
| 关键词: 贻贝、多糖类、分离和提纯、抗肿瘤药 |
| DOI：10.3724/SP.J.1008.2006.00998 |
| 投稿时间：2006-07-12修订日期：2006-08-31 |
| 基金项目:国家高技术研究发展计划/863计划(2002AA2Z3325);浙江省科技计划项目(2005ZJKJJH2202). |
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| Isolation and purification of water-soluble polysaccharide MP- Ⅰ from Mytilus coruscus and study on its in vitro anti-tumor activity |
| XU Hong-li,GUO Ting-ting,GUO Yi-feng,ZHANG Jian-peng,FENG Wei-hua,JIAO Bing-hua |
| (第二军医大学基础医学部生物化学与分子生物学教研室,上海,200433;上海水产大学生命学院中心实验室,上海,200090) |
| Abstract: |
| Objective：To separate and purify polysaccharide MP- I from Mytilus coruscus and to investigate its in vitro anti-tumor activity. Methods： The crude polysaccharide was extracted from Mytilus coruscus with hot water and the protein was removed using Sevage method. The polysaccharide MP- I was purified using DEAE-Sepharose and Sepharose CL-6B column chromatography. Monosaccharides analysis was carried out using thin-layer chromatography and gas chromatography. The in vitro anti-tumor activity of MP- I was assessed using MTT method. Results： The yield of MP- I was 2.14%. MP- I is mainly constituted by glucose. MP- I （at 0.5, 0. 1, 0.02 mg/ml） had different degrees of inhibitory effects on HO-8910, MCF-7, K562, and SMMC-7721 tumor cells in vitro（P〈0. 01）. The inhibitory rates of MP-I at 0. 5 mg/ml against HO-8910 and MCF-7 cells were 30.55 % and 36.38 %, respectively. Conclusion： Polysaccharide MP- I is mainly constituted by glucose and it has inhibitory effect on tumor cell lines HO-8910, MCF-7, K562, and SMMC-7721 in vitro |
| Key words: mussels polysaccharides isolation and purification, antineoplastic drugs |
