| 本文已被：浏览 2851次 下载 1982次 |
码上扫一扫！ |
| 雄激素非依赖性前列腺癌细胞亚系模型LNCaP-AI的建立 |
| 叶华茂,孙颖浩*,许传亮,王林辉,侯建国,高旭,杨庆,刘智勇 |
|
|
| (第二军医大学长海医院泌尿外科,上海 200433) |
|
| 摘要: |
| 目的：建立雄激素非依赖性前列腺癌细胞亚系模型LNCaP-AI。方法：长期在去雄激素的血清环境下培养雄激素依赖性LNCaP细胞，培养出能够适应无雄激素环境的LNCaP-AI细胞亚系。MTT、RT-PCR、免疫荧光方法观察LNCaP-AI细胞在无雄激素环境下的增殖能力和表达及分泌前列腺特异性抗原（PSA）的水平。结果：LNCaP前列腺癌细胞在无雄激素中培养3个月后逐渐适应了无雄激素的环境，成为雄激素非依赖的LNCaP-AI细胞亚系。LNCaP-AI细胞在无雄激素的环境下迅速增殖，能够分泌 PSA，但其PSA mRNA的表达水平是正常生长LNCaP细胞的44%。结论：成功构建雄激素非依赖性前列腺癌细胞亚系模型LNCaP-AI，可以模拟前列腺癌由雄激素依赖发展为雄激素非依赖的过程。 |
| 关键词: 前列腺癌 雄激素非依赖 LNCaP |
| DOI：10.3724/SP.J.1008.2008.00059 |
| 投稿时间：2007-04-17 |
| 基金项目: |
|
| Establishment of androgen-independent human prostate cancer cell line model LNCaP-AI |
| YE Hua-mao，SUN Ying-hao*,XU Chuan-liang,WANG Lin-hui,HOU Jian-guo,GAO Xu,YANG Qing,LIU Zhi-yong |
| (Department of Urology,Changhai Hospital,Second Military Medical University,Shanghai 200433, China) |
| Abstract: |
| Objective：To establish an androgen-independent human prostate cancer cell line model LNCaP-AI. Methods: LNCaP cells were cultured in absence of hormone for a long-term to establish LNCaP cell line LNCaP-AI, which can live without hormone. MTT, immunofluorescence and RT-PCR techniques were used to study the proliferation activity of LNCaP-AI cells and expression and secretion level of PSA by LNCaP-AI cells in absence of hormone.Results: After cultured for 3 months, LNCaP cells gradually became accustomed to the non-hormone condition, showing the characteristics of androgen-independent LNCaP-AI cell line. LNCaP-AI cells rapidly proliferated under the non-hormone condition and secreted PSA.However, PSA mRNA expression level in LNCaP-AI cells was 44% that of the LNCaP cells under hormone condition.Conclusion: Androgen-independent LNCaP-AI cell line may simulate the development of androgen-independence in prostate cancer cells and is an ideal model of androgen-independent prostate cancer cell line. |
| Key words: prostate carcinoma androgen-independent LNCaP |
