| 摘要: |
| 目的：观察肾癌组织中RASSF1A和BLU基因启动子甲基化状态，探讨二者在肾癌发生中的可能作用。方法：采用甲基化特异性PCR(MSP)技术检测26例肾癌组织和相应癌旁组织中RASSF1A和BLU基因启动子甲基化状态，分析二者检测结果。结果：肾癌组织中17例（65.4%）RASSF1A基因异常甲基化表达，相应癌旁组织中无RASSF1A基因异常甲基化表达；11例（42.3%）肾癌组织中BLU基因高甲基化，相应癌周组织中未发现BLU基因高甲基化。结论：肾癌组织中RASSF1A和BLU基因启动子高度甲基化，表明RASSF1A和BLU基因甲基化可能与肾癌的发生相关。 |
| 关键词: 肾肿瘤；基因，肿瘤抑制；DNA甲基化 RASSF1A BLU |
| DOI：10.3724/SP.J.1008.2007.01068 |
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| 基金项目:国家自然科学基金(30270522);上海市科委基金(054119604). |
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| Abnormal methylation of RASSF1A and BLU genes in renal carcinoma |
| DUAN Jianmin1, LI Zhi2*, ZHAO Min2, ZHANG Jianying2, PENG Bo1, HUANG Jianhua2 |
| (1.Department of Urology, The 10th People’s Hospital of Shanghai, Tongji University, Shanghai 200072, China;2.Department of Laboratory Medicine,the 10th People’s Hospital of Shanghai,Tongji University,Shanghai 200072) |
| Abstract: |
| Objective：To investigate the methylation status of the promoters of RASSF1A and BLU genes in the renal carcinoma tissues and to assess their roles in the tumorigenesis of renal carcinoma. Methods: Methylation specific PCR (MSP) method was used to examine the methylation status of the promoters of RASSF1A and BLU genes in the renal carcinoma tissues of 26 patients with renal carcinoma and their corresponding adjacent normal tissues; and the results were analyzed. Results: We found that 17 （65.4%） of the renal carcinoma tissues had abnormal methylation of RASSF1A gene, and there was no abnormal methylation of RASSF1A in the adjacent normal tissues. Eleven(42.3％) of 26 renal cancer tissues had hypermethylation of BLU gene, and there was no hypermethylation of BLU gene in the corresponding adjacent tissues. Conclusion: Hypermethylation of RASSF1A and BLU genes is present in the renal carcinoma tissues, suggesting the hypermethylation of RASSF1A and BLU might be associated with the tumorigenesis of renal carcinoma. |
| Key words: kidney neoplasms genes,tumor suppressor DNA methylation RASSF1A BLU |
