| 摘要: |
| 目的：从线粒体介导的内源性途径分析大鼠肝再生早期肝细胞凋亡执行酶caspase3激活的机制。方法：雄性SD大鼠35只,分成模型组15只（70%肝切除制作肝再生模型）、假手术组15只和正常对照组5只。模型组和假手术组根据时间分为术后3、6和24 h三个亚组,每个亚组5只。取各组大鼠肝组织,制备肝匀浆液。荧光法测肝组织凋亡酶caspase3和caspase9的活性；酶联免疫分析法测谷胱甘肽过氧化酶(GSHPx)活性；硝酸还原法测NO含量。结果：与假手术组相比,模型组凋亡酶caspase3和caspase9的活性在肝再生早期(术后3、6 h组)显著增高(P<0.01);GSHPx水平显著降低,NO分泌量显著增高(P<0.01)。结论：肝再生早期,肝组织处于氧化应激状态,线粒体介导的内源性途径参与凋亡执行酶的激活。 |
| 关键词: 肝再生 细胞凋亡 线粒体 caspase 3 caspase 9 氧化性应激 |
| DOI：10.3724/SP.J.1008.2007.01079 |
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| 基金项目:国家自然科学基金(30570837). |
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| Mechanism of mitochondria mediated activation of apoptotic enzyme caspase 3 during early phase of liver regeneration in rats |
| SHI Duo1,YUAN Bin1,YUAN Xing1,JIANG Hengyi1,SUN Xuejun2,MIAO Mingyong1*,JIAO Binghua1* |
| (1.Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China;2.Department of Diving Medicine Faculty of Naval Medicine,Second Military Medical University) |
| Abstract: |
| Objective：To analyze the activation mechanism of hepatic apoptosisrelated enzyme caspase3 during the early phase of liver regeneration in rats from the perspective of mitochondriamediated endogenous signal pathway.Methods: Thirtyfive male SD rats were randomly divided into 3 groups:model group \[70% partial hepatectomy (PH),n=15\],shamoperated group (n=15),and control group (n=5).The former 2 groups were further divided into 3 subgroups according to time after operation (3 h,6 h,and 24 h after operation,5 animals in each group).The animals in all groups were sacrificed and the liver homogenates were prepared.Using the fluorescence method,we determined the activities of hepatic caspase3 and caspase9 at 3 different time points after operation.The activity of glutathione peroxidase (GSHPx) and the content of NO were examined by enzyme immunoassay and nitrate reductase assay,respectively.Results: The activities of hepatic caspase3 and 9 both increased significantly compared with those of shamoperated group (P<0.01) at 3 h and 6 h after operation; the level of GSHPx was significantly decreased and the content of NO was significantly increased compared with those of the shamoperated group (P<0.01).Conclusion: Hepatic tissue is under oxidative stress during the early phase of liver regeneration,and the mitochondriamediated endogenous signal pathway is involved in the activation of caspase3. |
| Key words: liver regeneration apoptosis mitochondria caspase 3 caspase 9 oxidative stress |
